› 5hdm

X-ray diffraction
1.25Å resolution

Crystal structure of Arabidopsis thaliana glutamate-1-semialdehyde-2,1-aminomutase

Released:
DOI: 10.2210/pdb5hdm/pdb
PDB entry 5hdm coloured by chain, front view.
PDB entry 5hdm coloured by chain, side view.
PDB entry 5hdm coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Crystal structure of glutamate-1-semialdehyde-2,1-aminomutase from Arabidopsis thaliana.
Song Y, Pu H, Jiang T, Zhang L, Ouyang M
Acta Crystallogr F Struct Biol Commun 72 448-56 (2016)
PMID: 27303897

Function and Biology Details

Reaction catalysed: 
(S)-4-amino-5-oxopentanoate = 5-aminolevulinate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154885 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate-1-semialdehyde 2,1-aminomutase 1, chloroplastic Chains: A, B
Molecule details ›
Chains: A, B
Length: 434 amino acids
Theoretical weight: 46.41 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: P42799 (Residues: 41-474; Coverage: 92%)
Gene names: At5g63570, GSA1, HEML1, MBK5.3
Sequence domains: Aminotransferase class-III
Structure domains:

Ligands and Environments


Cofactor: Ligand PMP 2 x PMP

Cofactor: Ligand PLP 1 x PLP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 64.117Å b: 109.331Å c: 115.502Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.128 0.126 0.15
Expression system: Escherichia coli

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Citations

1 citations in other articles

A Single Nucleotide Substitution of GSAM Gene Causes Massive Accumulation of Glutamate 1-Semialdehyde and Yellow Leaf Phenotype in Rice.
Wang et al. (2021)

2 mentions without citation

Crystal structure of glutamate-1-semialdehyde-2,1-aminomutase from Arabidopsis thaliana.
Song et al. (2016)
1 more