› 5hdh

X-ray diffraction
2.6Å resolution

Crystal structure of human TLR8 with an uncleaved Z-loop

Released:
DOI: 10.2210/pdb5hdh/pdb
PDB entry 5hdh coloured by chain, front view.
PDB entry 5hdh coloured by chain, side view.
PDB entry 5hdh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Autoinhibition and relief mechanism by the proteolytic processing of Toll-like receptor 8.
Tanji H, Ohto U, Motoi Y, Shibata T, Miyake K, Shimizu T
Proc Natl Acad Sci U S A 113 3012-7 (2016)
PMID: 26929371

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192549 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Toll-like receptor 8 Chain: A
Molecule details ›
Chain: A
Length: 801 amino acids
Theoretical weight: 91.41 KDa
Source organism: Homo sapiens
Expression system: Drosophila
UniProt:
  • Canonical: Q9NR97 (Residues: 27-827; Coverage: 79%)
Gene names: TLR8, UNQ249/PRO286
Sequence domains: Leucine rich repeat
Structure domains: Ribonuclease Inhibitor

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
3 bound ligands:
Ligand NAG 6 x NAG
Ligand SO4 3 x SO4
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: R32
Unit cell:
a: 171.52Å b: 171.52Å c: 301.333Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.206 0.239
Expression system: Drosophila

Quick links

Citations

14 review citations

Toll-like Receptors and the Control of Immunity.
Fitzgerald et al. (2020)
13 more

3 mentions without citation

Structural and functional understanding of the toll-like receptors.
Asami et al. (2021)
2 more