Function and Biology Details
Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
- not assigned
Cellular component:
- not assigned
Sequence domains:
- DNA-directed DNA polymerase, family B
- DNA-directed DNA polymerase, family B, exonuclease domain
- DNA polymerase family B, thumb domain
- DNA-directed DNA polymerase, family B, conserved site
- DNA polymerase, palm domain superfamily
- DNA-directed DNA polymerase, family B, multifunctional domain
- DNA/RNA polymerase superfamily
- Ribonuclease H superfamily
2 more domains
Structure analysis Details
Assembly composition:
monomeric (preferred)
Assembly name:
Homing endonuclease PI-PspI (preferred)
PDBe Complex ID:
PDB-CPX-175915 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Homing endonuclease PI-PspI
Molecule details ›
Chain: A
Length: 775 amino acids
Theoretical weight: 90.72 KDa
Source organism: Pyrococcus
Expression system: Escherichia coli
UniProt:
Sequence domains:
Structure domains:
Length: 775 amino acids
Theoretical weight: 90.72 KDa
Source organism: Pyrococcus
Expression system: Escherichia coli
UniProt:
- Canonical:
Q51334 (Residues: 1-1312; Coverage: 59%)
Sequence domains:
Structure domains:
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
PHOTON FACTORY BEAMLINE BL-5A
Spacegroup:
P212121
Expression system: Escherichia coli
