› 5gz2

X-ray diffraction
2Å resolution

luciferase complex with 7-cy-L

Released:
DOI: 10.2210/pdb5gz2/pdb
PDB entry 5gz2 coloured by chain, front view.
PDB entry 5gz2 coloured by chain, side view.
PDB entry 5gz2 coloured by chain, top view.
Source organism: Photinus pyralis
Entry authors: Su J, Wang F, Gu L

Function and Biology Details

Reaction catalysed: 
D-firefly luciferin + O(2) + ATP = firefly oxyluciferin + CO(2) + AMP + diphosphate + light
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140262 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Luciferin 4-monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 436 amino acids
Theoretical weight: 48.53 KDa
Source organism: Photinus pyralis
Expression system: Escherichia coli
UniProt:
  • Canonical: P08659 (Residues: 3-438; Coverage: 79%)
Sequence domains: AMP-binding enzyme
Structure domains: N-terminal domain of ligase-like

Ligands and Environments

1 bound ligand:
Ligand 7BV 1 x 7BV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 12.3.1
Spacegroup: P41
Unit cell:
a: 74.154Å b: 74.154Å c: 95.622Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.208 0.239
Expression system: Escherichia coli

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