› 5gxi

X-ray diffraction
1.85Å resolution

Structure of the Gemin5 WD40 domain in complex with AAUUUUUGAG

Released:
DOI: 10.2210/pdb5gxi/pdb
PDB entry 5gxi coloured by chain, front view.
PDB entry 5gxi coloured by chain, side view.
PDB entry 5gxi coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into Gemin5-guided selection of pre-snRNAs for snRNP assembly.
Xu C, Ishikawa H, Izumikawa K, Li L, He H, Nobe Y, Yamauchi Y, Shahjee HM, Wu XH, Yu YT, Isobe T, Takahashi N, Min J
Genes Dev 30 2376-2390 (2016)
PMID: 27881600

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186249 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Gem-associated protein 5 Chain: A
Molecule details ›
Chain: A
Length: 757 amino acids
Theoretical weight: 84.34 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8TEQ6 (Residues: 1-739; Coverage: 49%)
Gene name: GEMIN5
Sequence domains:
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
RNA (5'-R(*A*AP*UP*UP*UP*UP*UP*GP*AP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 10 nucleotides
Theoretical weight: 3.16 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand UNX 22 x UNX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E DW
Spacegroup: P21
Unit cell:
a: 57.85Å b: 124.52Å c: 60.6Å
α: 90° β: 116.77° γ: 90°
R-values:
R R work R free
0.166 0.164 0.207
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

7 review citations

Insights into Structural and Mechanistic Features of Viral IRES Elements.
Martinez-Salas et al. (2017)
6 more

3 mentions without citation

Control of the eIF4E activity: structural insights and pharmacological implications.
Romagnoli et al. (2021)
2 more