› 5goy

X-ray diffraction
2.28Å resolution

The crystal structure of human cytosolic methionyl-tRNA synthetase in complex with methionine

Released:
DOI: 10.2210/pdb5goy/pdb
PDB entry 5goy coloured by chain, front view.
PDB entry 5goy coloured by chain, side view.
PDB entry 5goy coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Lee HJ, Cho HY, Kang BS

Function and Biology Details

Reaction catalysed: 
ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157412 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methionine--tRNA ligase, cytoplasmic Chain: A
Molecule details ›
Chain: A
Length: 624 amino acids
Theoretical weight: 71.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P56192 (Residues: 221-834; Coverage: 68%)
Gene names: MARS, MARS1
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand MET 1 x MET
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P212121
Unit cell:
a: 65.539Å b: 93.133Å c: 122.482Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.196 0.242
Expression system: Escherichia coli BL21(DE3)

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