› 5fjt

X-ray diffraction
2.11Å resolution

N-acyl amino acid racemase from Amycolatopsis sp. Ts-1-60: G291D F323 mutant in complex with N-acetyl phenylalanine

Released:
DOI: 10.2210/pdb5fjt/pdb
PDB entry 5fjt coloured by chain, front view.
PDB entry 5fjt coloured by chain, side view.
PDB entry 5fjt coloured by chain, top view.
Source organism: Amycolatopsis sp.
Entry authors: Sanchez Carron G, Campopiano D, Grogan G

Function and Biology Details

Reaction catalysed: 
(1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate = 2-succinylbenzoate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175018 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-succinylamino acid racemase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 368 amino acids
Theoretical weight: 39.52 KDa
Source organism: Amycolatopsis sp.
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q44244 (Residues: 1-368; Coverage: 100%)
Gene name: Aaar
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand 5CR 4 x 5CR
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: R32
Unit cell:
a: 216.204Å b: 216.204Å c: 258.691Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.161 0.16 0.181
Expression system: Escherichia coli BL21(DE3)

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