› 5exx

X-ray diffraction
3.31Å resolution

AAA+ ATPase FleQ from Pseudomonas aeruginosa bound to c-di-GMP

Released:
DOI: 10.2210/pdb5exx/pdb
PDB entry 5exx coloured by chain, front view.
PDB entry 5exx coloured by chain, side view.
PDB entry 5exx coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Mechanistic insights into c-di-GMP-dependent control of the biofilm regulator FleQ from Pseudomonas aeruginosa.
Matsuyama BY, Krasteva PV, Baraquet C, Harwood CS, Sondermann H, Navarro MV
Proc Natl Acad Sci U S A 113 E209-18 (2016)
PMID: 26712005

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124382 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional regulator FleQ Chain: A
Molecule details ›
Chain: A
Length: 343 amino acids
Theoretical weight: 38.38 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G3XCV0 (Residues: 137-477; Coverage: 70%)
Gene names: PA1097, fleQ
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand C2E 2 x C2E
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P6322
Unit cell:
a: 137.836Å b: 137.836Å c: 172.773Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.264 0.263 0.29
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 review citations

Cyclic di-GMP: second messenger extraordinaire.
Jenal et al. (2017)
18 more

5 mentions without citation

Cyclic di-AMP, a second messenger of primary importance: tertiary structures and binding mechanisms.
He et al. (2020)
4 more