› 5eny

X-ray diffraction
4Å resolution

Ketosynthase from module 6 connected to acyl carrier protein from module 5 (unobservable) of the bacillaene synthase from Bacillus subtilis 168

Released:
DOI: 10.2210/pdb5eny/pdb
PDB entry 5eny coloured by chain, front view.
PDB entry 5eny coloured by chain, side view.
PDB entry 5eny coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
The LINKS motif zippers trans-acyltransferase polyketide synthase assembly lines into a biosynthetic megacomplex.
Gay DC, Wagner DT, Meinke JL, Zogzas CE, Gay GR, Keatinge-Clay AT
J Struct Biol 193 196-205 (2016)
PMID: 26724270

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo octamer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170206 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyketide synthase PksL Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 764 amino acids
Theoretical weight: 85.82 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: Q05470 (Residues: 2719-3462; Coverage: 16%)
Gene names: BSU17190, outG, pksA, pksL, pksX
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P1
Unit cell:
a: 63.106Å b: 112.732Å c: 211.441Å
α: 104.96° β: 90.07° γ: 106.32°
R-values:
R R work R free
0.335 0.333 0.354
Expression system: Escherichia coli

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Citations

6 review citations

The Uncommon Enzymology of Cis-Acyltransferase Assembly Lines.
Keatinge-Clay AT. (2017)
5 more