› 5elv

X-ray diffraction
1.92Å resolution

Crystal structure of the GluA2 ligand-binding domain (S1S2J-L504-N775) in complex with glutamate and BPAM-521 at 1.92 A resolution

Released:
DOI: 10.2210/pdb5elv/pdb
PDB entry 5elv coloured by chain, front view.
PDB entry 5elv coloured by chain, side view.
PDB entry 5elv coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Enthalpy-Entropy Compensation in the Binding of Modulators at Ionotropic Glutamate Receptor GluA2.
Krintel C, Francotte P, Pickering DS, Juknaitė L, Pøhlsgaard J, Olsen L, Frydenvang K, Goffin E, Pirotte B, Kastrup JS
Biophys J 110 2397-2406 (2016)
PMID: 27276258

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148607 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate receptor 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 264 amino acids
Theoretical weight: 29.3 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19491 (Residues: 413-527, 653-797; Coverage: 30%)
Gene names: GluA2, Glur2, Gria2
Sequence domains:
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

7 bound ligands:
Ligand SO4 4 x SO4
Ligand GOL 4 x GOL
Ligand CL 3 x CL
Ligand ACT 9 x ACT
Ligand 5PX 2 x 5PX
Ligand GLU 2 x GLU
Ligand PEG 2 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: P21212
Unit cell:
a: 98.79Å b: 122.48Å c: 47.54Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.162 0.209
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

The Molecular Origin of Enthalpy/Entropy Compensation in Biomolecular Recognition.
Fox et al. (2018)
1 more