› 5eg5

X-ray diffraction
1.97Å resolution

As (III) S-adenosylmethyltransferase cysteine mutant C72A bound Phenylarsine oxide (PhAs(III) in the arsenic binding site

Released:
DOI: 10.2210/pdb5eg5/pdb
PDB entry 5eg5 coloured by chain, front view.
PDB entry 5eg5 coloured by chain, side view.
PDB entry 5eg5 coloured by chain, top view.
Source organism: Cyanidioschyzon sp. 5508
Entry authors: Packianathan C, Marapakala K, Kandavelu P, Rosen BP

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + arsenite = S-adenosyl-L-homocysteine + methylarsonate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110992 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyltransferase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 377 amino acids
Theoretical weight: 41.77 KDa
Source organism: Cyanidioschyzon sp. 5508
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C0JV69 (Residues: 1-370; Coverage: 93%)
Gene name: arsM
Sequence domains: Methyltransferase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand PA0 1 x PA0
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 85.255Å b: 47.369Å c: 100.261Å
α: 90° β: 113.59° γ: 90°
R-values:
R R work R free
0.198 0.195 0.25
Expression system: Escherichia coli BL21(DE3)

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