› 5ea9

X-ray diffraction
1.71Å resolution

Crystal Structure of Trypanosoma cruzi Dihydroorotate Dehydrogenase in Complex with Neq0130

Released:
DOI: 10.2210/pdb5ea9/pdb
PDB entry 5ea9 coloured by chain, front view.
PDB entry 5ea9 coloured by chain, side view.
PDB entry 5ea9 coloured by chain, top view.
Source organism: Trypanosoma cruzi strain CL Brener
Entry authors: Rocha JR, Inaoka DK, Cheleski J, Shiba T, Harada S, Montanari CA, Kita K

Function and Biology Details

Reaction catalysed: 
(S)-dihydroorotate + fumarate = orotate + succinate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175459 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydroorotate dehydrogenase (fumarate) Chains: A, B
Molecule details ›
Chains: A, B
Length: 314 amino acids
Theoretical weight: 34.15 KDa
Source organism: Trypanosoma cruzi strain CL Brener
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4D3W2 (Residues: 2-314; Coverage: 100%)
Gene names: Tc00.1047053508375.50, pyr4, tcdhod2
Sequence domains: Dihydroorotate dehydrogenase
Structure domains:

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN
4 bound ligands:
Ligand 5LM 2 x 5LM
Ligand GOL 13 x GOL
Ligand EDO 11 x EDO
Ligand NCO 2 x NCO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: P212121
Unit cell:
a: 68.284Å b: 71.863Å c: 124.264Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.179 0.221
Expression system: Escherichia coli BL21(DE3)

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