› 5e9e

X-ray diffraction
2.4Å resolution

Crystal Structure of the Alpha-kinase Domain of Myosin-II Heavy Chain Kinase A in Complex with AMP-PNP

Released:
DOI: 10.2210/pdb5e9e/pdb
PDB entry 5e9e coloured by chain, front view.
PDB entry 5e9e coloured by chain, side view.
PDB entry 5e9e coloured by chain, top view.
Source organism: Dictyostelium discoideum
Primary publication:
Structure of the Dictyostelium Myosin-II Heavy Chain Kinase A (MHCK-A) α-kinase domain apoenzyme reveals a novel autoinhibited conformation.
Ye Q, Yang Y, van Staalduinen L, Crawley SW, Liu L, Brennan S, Côté GP, Jia Z
Sci Rep 6 26634 (2016)
PMID: 27211275

Function and Biology Details

Reaction catalysed: 
ATP + [myosin heavy-chain] = ADP + [myosin heavy-chain] phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154659 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myosin heavy chain kinase A Chains: A, B
Molecule details ›
Chains: A, B
Length: 307 amino acids
Theoretical weight: 34.53 KDa
Source organism: Dictyostelium discoideum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42527 (Residues: 552-841; Coverage: 25%)
Gene names: DDB_G0291231, mhckA, mhkA
Sequence domains: Alpha-kinase family
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ANP 2 x ANP
Ligand ZN 2 x ZN
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P21212
Unit cell:
a: 82.601Å b: 110.112Å c: 78.265Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.191 0.236
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 citation in other articles

Mass Spectrometric Analysis of TRPM6 and TRPM7 Phosphorylation Reveals Regulatory Mechanisms of the Channel-Kinases.
Cai et al. (2017)
4 more

1 mention without citation

eEF2K Inhibitor Design: The Progression of Exemplary Structure-Based Drug Design.
Klupt et al. (2023)