› 5dzq

X-ray diffraction
1.89Å resolution

C3larvin toxin, an ADP-ribosyltransferase from Paenibacillus larvae, Orthorhombic Form

Released:
DOI: 10.2210/pdb5dzq/pdb
PDB entry 5dzq coloured by chain, front view.
PDB entry 5dzq coloured by chain, side view.
PDB entry 5dzq coloured by chain, top view.
Source organism: Paenibacillus larvae subsp. larvae BRL-230010
Primary publication:
Structural variability of C3larvin toxin. Intrinsic dynamics of the α/β fold of the C3-like group of mono-ADP-ribosyltransferase toxins.
Lugo MR, Ravulapalli R, Dutta D, Merrill AR
J Biomol Struct Dyn 34 2537-2560 (2016)
PMID: 26610041

Function and Biology Details

Reaction catalysed: 
NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197728 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ADP ribosyltransferase domain-containing protein Chains: A, O
Molecule details ›
Chains: A, O
Length: 213 amino acids
Theoretical weight: 24.7 KDa
Source organism: Paenibacillus larvae subsp. larvae BRL-230010
Expression system: Escherichia coli
UniProt:
  • Canonical: W2E3J5 (Residues: 2-190; Coverage: 100%)
Gene name: ERIC1_2c04930
Sequence domains: ADP-ribosyltransferase exoenzyme
Structure domains: Toxin ADP-ribosyltransferase; Chain A, domain 1

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 61.073Å b: 76.002Å c: 89.367Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.23
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Development of Anti-Virulence Therapeutics against Mono-ADP-Ribosyltransferase Toxins.
Lugo et al. (2020)
3 other articles

1 mention without citation

A Structural Approach to Anti-Virulence: A Discovery Pipeline.
McCarthy et al. (2021)