› 5dtw

X-ray diffraction
2.44Å resolution

Function and Biology Details

Reaction catalysed: 
(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161873 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable enoyl-CoA hydratase EchA6 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 263 amino acids
Theoretical weight: 28.23 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WNP1 (Residues: 1-243; Coverage: 100%)
Gene names: MTCY31.33, Rv0905, echA6
Sequence domains: Enoyl-CoA hydratase/isomerase
Structure domains: 2-enoyl-CoA Hydratase; Chain A, domain 1

Ligands and Environments

1 bound ligand:
Ligand 5F9 2 x 5F9
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 113.603Å b: 51.275Å c: 156.825Å
α: 90° β: 106.8° γ: 90°
R-values:
R R work R free
0.224 0.222 0.275
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Mycobacterial cell wall biosynthesis: a multifaceted antibiotic target.
Abrahams et al. (2018)
9 more