› 5dpq

X-ray diffraction
1.78Å resolution

Crystal Structure of E72A mutant of domain swapped dimer Human Cellular Retinol Binding Protein

Released:
DOI: 10.2210/pdb5dpq/pdb
PDB entry 5dpq coloured by chain, front view.
PDB entry 5dpq coloured by chain, side view.
PDB entry 5dpq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Domain-Swapped Dimers of Intracellular Lipid-Binding Proteins: Evidence for Ordered Folding Intermediates.
Assar Z, Nossoni Z, Wang W, Santos EM, Kramer K, McCornack C, Vasileiou C, Borhan B, Geiger JH
Structure 24 1590-8 (2016)
PMID: 27524203

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156130 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Retinol-binding protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 133 amino acids
Theoretical weight: 15.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P50120 (Residues: 2-134; Coverage: 99%)
Gene names: CRBP2, RBP2
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

1 bound ligand:
Ligand ACT 4 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P212121
Unit cell:
a: 62.238Å b: 72.901Å c: 60.487Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.174 0.22
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Protein Assembly by Design.
Zhu et al. (2021)
4 other articles

1 mention without citation

Domain-Swapped Dimers of Intracellular Lipid-Binding Proteins: Evidence for Ordered Folding Intermediates.
Assar et al. (2016)