Function and Biology Details
Biochemical function:
Biological process:
Cellular component:
Structure analysis Details
Assembly composition:
hetero octamer (preferred)
Assembly name:
Cas1-Cas2 complex and DNA (preferred)
PDBe Complex ID:
PDB-CPX-118575 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
CRISPR-associated endonuclease Cas1
Molecule details ›
Chains: A, B, C, D
Length: 280 amino acids
Theoretical weight: 30.71 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: CRISPR associated protein Cas1
Structure domains:
Length: 280 amino acids
Theoretical weight: 30.71 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
Q46896 (Residues: 2-281; Coverage: 92%)
Sequence domains: CRISPR associated protein Cas1
Structure domains:
CRISPR-associated endoribonuclease Cas2
Molecule details ›
Chains: E, F
Length: 78 amino acids
Theoretical weight: 8.8 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
Sequence domains: CRISPR-associated protein (Cas_Cas2CT1978)
Length: 78 amino acids
Theoretical weight: 8.8 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
- Canonical: P45956 (Residues: 1-78; Coverage: 83%)
Sequence domains: CRISPR-associated protein (Cas_Cas2CT1978)
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
SSRF BEAMLINE BL17U
Spacegroup:
P212121
Expression systems:
- Escherichia coli BL21(DE3)
- Escherichia coli
