› 5dlh

X-ray diffraction
2.25Å resolution

SFX structure of thermolysin

Released:
DOI: 10.2210/pdb5dlh/pdb
PDB entry 5dlh coloured by chain, front view.
PDB entry 5dlh coloured by chain, side view.
PDB entry 5dlh coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Entry authors: DeMirci HD, Sauter NK, Brewster AS, Kern J

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: -|-Leu > -|-Phe.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154956 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thermolysin Chain: A
Molecule details ›
Chain: A
Length: 316 amino acids
Theoretical weight: 34.36 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P43133 (Residues: 236-551; Coverage: 61%)
Gene names: nprM, nprS
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLAC LCLS BEAMLINE CXI
Spacegroup: P6122
Unit cell:
a: 93.794Å b: 93.794Å c: 132.243Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.179 0.227
Expression system: Escherichia coli

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