› 5dhm

X-ray diffraction
1.9Å resolution

Crystal structure of the fimbrial protein Mfa4 from Porphyromonas gingivalis

Released:
DOI: 10.2210/pdb5dhm/pdb
PDB entry 5dhm coloured by chain, front view.
PDB entry 5dhm coloured by chain, side view.
PDB entry 5dhm coloured by chain, top view.
Source organism: Porphyromonas gingivalis ATCC 33277
Primary publication:
Structure of the fimbrial protein Mfa4 from Porphyromonas gingivalis in its precursor form: implications for a donor-strand complementation mechanism.
Kloppsteck P, Hall M, Hasegawa Y, Persson K
Sci Rep 6 22945 (2016)
PMID: 26972441

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109701 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Minor fimbrium tip subunit MfA4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 53 amino acids
Theoretical weight: 6.46 KDa
Source organism: Porphyromonas gingivalis ATCC 33277
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: B2RHG4 (Residues: 26-51; Coverage: 8%)
Gene name: PGN_0290
Minor fimbrium tip subunit MfA4 Chains: C, D
Molecule details ›
Chains: C, D
Length: 282 amino acids
Theoretical weight: 31.49 KDa
Source organism: Porphyromonas gingivalis ATCC 33277
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: B2RHG4 (Residues: 52-333; Coverage: 90%)
Gene name: PGN_0290
Sequence domains: Major fimbrial subunit protein (FimA)
Structure domains: Immunoglobulin-like

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 54.683Å b: 84.536Å c: 138.364Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.23
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

A comprehensive guide to pilus biogenesis in Gram-negative bacteria.
Hospenthal et al. (2017)
1 more

2 mentions without citation

Structure of the fimbrial protein Mfa4 from Porphyromonas gingivalis in its precursor form: implications for a donor-strand complementation mechanism.
Kloppsteck et al. (2016)
1 more