› 5dfx

X-ray diffraction
1.8Å resolution

Structure of the parental state of GAF3 from Slr1393 of Synechocystis sp. PCC6803 (in vivo assembled protein/chromophore)

Released:
DOI: 10.2210/pdb5dfx/pdb
PDB entry 5dfx coloured by chain, front view.
PDB entry 5dfx coloured by chain, side view.
PDB entry 5dfx coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6803 substr. Kazusa
Primary publication:
Structural elements regulating the photochromicity in a cyanobacteriochrome
Xu X-L, Hoeppner A, Wiebeler C, Zhao K-H, Schapiro I, Gaertner W
Proc. Natl. Acad. Sci. U.S.A. (2020)

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159882 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
histidine kinase Chain: A
Molecule details ›
Chain: A
Length: 215 amino acids
Theoretical weight: 24.86 KDa
Source organism: Synechocystis sp. PCC 6803 substr. Kazusa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P73184 (Residues: 441-597; Coverage: 16%)
Gene name: slr1393
Sequence domains: GAF domain
Structure domains: Beta-Lactamase

Ligands and Environments

1 bound ligand:
Ligand CYC 1 x CYC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P41212
Unit cell:
a: 63.12Å b: 63.12Å c: 118.83Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.164 0.199
Expression system: Escherichia coli BL21(DE3)

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