› 5det

X-ray diffraction
1.95Å resolution

X-ray structure of human RBPMS in complex with the RNA

Released:
DOI: 10.2210/pdb5det/pdb
PDB entry 5det coloured by chain, front view.
PDB entry 5det coloured by chain, side view.
PDB entry 5det coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis underlying CAC RNA recognition by the RRM domain of dimeric RNA-binding protein RBPMS.
Teplova M, Farazi TA, Tuschl T, Patel DJ
Q Rev Biophys 49 e1 (2016)
PMID: 26347403

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187978 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
RNA-binding protein with multiple splicing Chains: A, B
Molecule details ›
Chains: A, B
Length: 98 amino acids
Theoretical weight: 11.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93062 (Residues: 14-111; Coverage: 50%)
Gene names: HERMES, RBPMS
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
RNA (5'-R(P*UP*CP*AP*CP*U)-3') Chain: Q
Molecule details ›
Chain: Q
Length: 5 nucleotides
Theoretical weight: 1.51 KDa
Source organism: Homo sapiens
Expression system: Not provided
RNA (5'-R(*UP*CP*AP*C)-3') Chain: P
Molecule details ›
Chain: P
Length: 4 nucleotides
Theoretical weight: 1.2 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21
Unit cell:
a: 30.8Å b: 90.232Å c: 34.16Å
α: 90° β: 93.7° γ: 90°
R-values:
R R work R free
0.192 0.19 0.24
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Stress granules, RNA-binding proteins and polyglutamine diseases: too much aggregation?
Marcelo et al. (2021)
1 more

4 mentions without citation

Structural basis underlying CAC RNA recognition by the RRM domain of dimeric RNA-binding protein RBPMS.
Teplova et al. (2016)
3 more