› 5d9w

X-ray diffraction
1.69Å resolution

Dehydroascorbate reductase (OsDHAR) complexed with ASA

Released:
DOI: 10.2210/pdb5d9w/pdb
PDB entry 5d9w coloured by chain, front view.
PDB entry 5d9w coloured by chain, side view.
PDB entry 5d9w coloured by chain, top view.
Source organism: Oryza sativa Japonica Group
Primary publication:
Structural understanding of the recycling of oxidized ascorbate by dehydroascorbate reductase (OsDHAR) from Oryza sativa L. japonica.
Do H, Kim IS, Jeon BW, Lee CW, Park AK, Wi AR, Shin SC, Park H, Kim YS, Yoon HS, Kim HW, Lee JH
Sci Rep 6 19498 (2016)
PMID: 26775680

Function and Biology Details

Reactions catalysed: 
2 glutathione + dehydroascorbate = glutathione disulfide + ascorbate
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179230 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable glutathione S-transferase DHAR1, cytosolic Chain: A
Molecule details ›
Chain: A
Length: 230 amino acids
Theoretical weight: 25.63 KDa
Source organism: Oryza sativa Japonica Group
Expression system: Escherichia coli
UniProt:
  • Canonical: Q65XA0 (Residues: 1-213; Coverage: 100%)
Gene names: DHAR, DHAR1, LOC_Os05g02530, OJ1654_B10.18, Os05g0116100, P0496H07.8
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ASC 1 x ASC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P21
Unit cell:
a: 46.848Å b: 47.279Å c: 50.992Å
α: 90° β: 108.23° γ: 90°
R-values:
R R work R free
0.204 0.203 0.237
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Regulation of Ascorbate-Glutathione Pathway in Mitigating Oxidative Damage in Plants under Abiotic Stress.
Hasanuzzaman et al. (2019)
5 more

1 mention without citation

A Computational Study of the Role of Secondary Metabolites for Mitigation of Acid Soil Stress in Cereals Using Dehydroascorbate and Mono-Dehydroascorbate Reductases.
Choudhury et al. (2022)