› 5d8y

X-ray diffraction
2.05Å resolution

2.05A resolution structure of iron bound BfrB (L68A E81A) from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb5d8y/pdb
PDB entry 5d8y coloured by chain, front view.
PDB entry 5d8y coloured by chain, side view.
PDB entry 5d8y coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Characterization of the Bacterioferritin/Bacterioferritin Associated Ferredoxin Protein-Protein Interaction in Solution and Determination of Binding Energy Hot Spots.
Wang Y, Yao H, Cheng Y, Lovell S, Battaile KP, Midaugh CR, Rivera M
Biochemistry 54 6162-75 (2015)
PMID: 26368531

Function and Biology Details

Reaction catalysed: 
4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191138 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacterioferritin Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X
Length: 158 amino acids
Theoretical weight: 18.48 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HY79 (Residues: 1-158; Coverage: 100%)
Gene names: PA3531, bfrB
Sequence domains: Ferritin-like domain
Structure domains: Ferritin

Ligands and Environments

4 bound ligands:
Ligand FE2 144 x FE2
Ligand ACT 24 x ACT
Ligand K 6 x K
Ligand HEM 12 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P32
Unit cell:
a: 125.845Å b: 125.845Å c: 272.518Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.153 0.151 0.196
Expression system: Escherichia coli

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Citations

2 review citations

Ferritins: furnishing proteins with iron.
Bradley et al. (2016)
1 more