› 5czv

X-ray diffraction
3.19Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-230403 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Notch 3 extracellular truncation Chain: A
Molecule details ›
Chain: A
Length: 271 amino acids
Theoretical weight: 30.1 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9UM47 (Residues: 1378-1640; Coverage: 12%)
Gene name: NOTCH3
Sequence domains:
Structure domains:
Fab 20350 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 223 amino acids
Theoretical weight: 23.79 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Fab 20350 light chain Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.56 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand CA 3 x CA
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: C2
Unit cell:
a: 91.917Å b: 104.349Å c: 92.849Å
α: 90° β: 113.17° γ: 90°
R-values:
R R work R free
0.206 0.204 0.256
Expression system: Homo sapiens

Quick links

Citations

26 review citations

Notch Signaling in Development, Tissue Homeostasis, and Disease.
Siebel et al. (2017)
25 more

2 mentions without citation

Characterization of activating mutations of NOTCH3 in T-cell acute lymphoblastic leukemia and anti-leukemic activity of NOTCH3 inhibitory antibodies.
Bernasconi-Elias et al. (2016)
1 more