› 5ckt

X-ray diffraction
2Å resolution

Crystal Structure of KorA, a plasmid-encoded, global transcription regulator

Released:
DOI: 10.2210/pdb5ckt/pdb
PDB entry 5ckt coloured by chain, front view.
PDB entry 5ckt coloured by chain, side view.
PDB entry 5ckt coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Flexibility of KorA, a plasmid-encoded, global transcription regulator, in the presence and the absence of its operator.
Rajasekar KV, Lovering AL, Dancea F, Scott DJ, Harris SA, Bingle LE, Roessle M, Thomas CM, Hyde EI, White SA
Nucleic Acids Res 44 4947-56 (2016)
PMID: 27016739

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-136493 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
TrfB transcriptional repressor protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 99 amino acids
Theoretical weight: 11.04 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03052 (Residues: 1-99; Coverage: 98%)
Gene names: korA, trfB
Sequence domains: TrfB plasmid transcriptional repressor
Structure domains: Arc Repressor Mutant, subunit A
TrfB transcriptional repressor protein Chain: D
Molecule details ›
Chain: D
Length: 101 amino acids
Theoretical weight: 11.32 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03052 (Residues: 1-101; Coverage: 100%)
Gene names: korA, trfB
Sequence domains: TrfB plasmid transcriptional repressor

Ligands and Environments

1 bound ligand:
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P1
Unit cell:
a: 42.58Å b: 49.69Å c: 52.33Å
α: 98.09° β: 93.87° γ: 106.01°
R-values:
R R work R free
0.197 0.196 0.222
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

Intrinsic disorder in the partitioning protein KorB persists after co-operative complex formation with operator DNA and KorA.
Hyde et al. (2017)
2 more

1 mention without citation

Flexibility of KorA, a plasmid-encoded, global transcription regulator, in the presence and the absence of its operator.
Rajasekar et al. (2016)