› 5ckm

X-ray diffraction
2.73Å resolution

The CUB1-EGF-CUB2 domains of rat MBL-associated serine protease-2 (MASP-2) bound to Ca2+

Released:
DOI: 10.2210/pdb5ckm/pdb
PDB entry 5ckm coloured by chain, front view.
PDB entry 5ckm coloured by chain, side view.
PDB entry 5ckm coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Flexibility in Mannan-Binding Lectin-Associated Serine Proteases-1 and -2 Provides Insight on Lectin Pathway Activation.
Nan R, Furze CM, Wright DW, Gor J, Wallis R, Perkins SJ
Structure 25 364-375 (2017)
PMID: 28111019

Function and Biology Details

Reaction catalysed: 
Selective cleavage after Arg(223) in complement component C2 (-Ser-Leu-Gly-Arg-|-Lys-Ile-Gln-Ile) and after Arg(76) in complement component C4 (-Gly-Leu-Gln-Arg-|-Ala-Leu-Glu-Ile)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191620 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mannan-binding lectin serine protease 2 A chain Chain: A
Molecule details ›
Chain: A
Length: 277 amino acids
Theoretical weight: 31.4 KDa
Source organism: Rattus norvegicus
Expression system: Cricetulus griseus
UniProt:
  • Canonical: Q9JJS8 (Residues: 21-297; Coverage: 42%)
Gene name: Masp2
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 3 x CA
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2221
Unit cell:
a: 82.9Å b: 119.39Å c: 104.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.204 0.266
Expression system: Cricetulus griseus

Quick links

Citations

2 review citations

Complement in neurological disorders and emerging complement-targeted therapeutics.
Dalakas et al. (2020)
1 more

2 mentions without citation

Structure of the C1r-C1s interaction of the C1 complex of complement activation.
Almitairi et al. (2018)
1 more