› 5cae

X-ray diffraction
2.2Å resolution

Succinate bound to pig GTP-specific succinyl-CoA synthetase

Released:
DOI: 10.2210/pdb5cae/pdb
PDB entry 5cae coloured by chain, front view.
PDB entry 5cae coloured by chain, side view.
PDB entry 5cae coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Structural basis for the binding of succinate to succinyl-CoA synthetase.
Huang J, Fraser ME
Acta Crystallogr D Struct Biol 72 912-21 (2016)
PMID: 27487822

Function and Biology Details

Reactions catalysed: 
GTP + succinate + CoA = GDP + phosphate + succinyl-CoA
ATP + succinate + CoA = ADP + phosphate + succinyl-CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127591 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 313 amino acids
Theoretical weight: 33.13 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O19069 (Residues: 42-346; Coverage: 88%)
Gene name: SUCLG1
Sequence domains:
Structure domains:
Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial Chain: B
Molecule details ›
Chain: B
Length: 395 amino acids
Theoretical weight: 42.67 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P53590 (Residues: 39-433; Coverage: 91%)
Gene name: SUCLG2
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand COA 1 x COA
5 bound ligands:
Ligand CL 1 x CL
Ligand GOL 2 x GOL
Ligand PO4 1 x PO4
Ligand MG 1 x MG
Ligand SIN 1 x SIN
1 modified residue:
Ligand CSS 1 x CSS

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08B1-1
Spacegroup: P21
Unit cell:
a: 86.67Å b: 81.02Å c: 50.78Å
α: 90° β: 104.9° γ: 90°
R-values:
R R work R free
0.17 0.165 0.214
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Lysine succinylation, the metabolic bridge between cancer and immunity.
Shen et al. (2023)
1 more

5 mentions without citation

Magnetic Resonance Imaging Score and Classification System (AMADEUS) for Assessment of Preoperative Cartilage Defect Severity.
Jungmann et al. (2017)
4 more