› 5c18

X-ray diffraction
3.3Å resolution

p97-delta709-728 in complex with ATP-gamma-S

Released:
DOI: 10.2210/pdb5c18/pdb
PDB entry 5c18 coloured by chain, front view.
PDB entry 5c18 coloured by chain, side view.
PDB entry 5c18 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Basis of ATP Hydrolysis and Intersubunit Signaling in the AAA+ ATPase p97.
Hänzelmann P, Schindelin H
Structure 24 127-139 (2016)
PMID: 26712278

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157184 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transitional endoplasmic reticulum ATPase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 785 amino acids
Theoretical weight: 86.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P55072 (Residues: 2-806; Coverage: 97%)
Gene names: HEL-220, HEL-S-70, VCP
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand AGS 12 x AGS
Ligand MG 12 x MG
Ligand CL 6 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P212121
Unit cell:
a: 140.33Å b: 180.059Å c: 255.612Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.211 0.249
Expression system: Escherichia coli BL21

Quick links

Citations

12 review citations

Structure and function of the AAA+ ATPase p97/Cdc48p.
Xia et al. (2016)
11 more

15 mentions without citation

The Interplay of Cofactor Interactions and Post-translational Modifications in the Regulation of the AAA+ ATPase p97.
Hänzelmann et al. (2017)
14 more