5but

X-ray diffraction
5.97Å resolution

Crystal structure of inactive conformation of KtrAB K+ transporter

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero hexamer (preferred)
homo dimer
PDBe Complex ID:
PDB-CPX-128408 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ktr system potassium uptake protein A Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 288 amino acids
Theoretical weight: 32.04 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O32080 (Residues: 1-144, 149-156; Coverage: 69%)
Gene names: BSU31090, ktrA, yuaA
Sequence domains: TrkA-N domain
Ktr system potassium uptake protein B Chains: I, J, K, L
Molecule details ›
Chains: I, J, K, L
Length: 445 amino acids
Theoretical weight: 48.16 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O32081 (Residues: 1-445; Coverage: 100%)
Gene names: BSU31100, ktrB, yubG
Sequence domains: Cation transport protein

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: C2
Unit cell:
a: 307.06Å b: 79.41Å c: 205.65Å
α: 90° β: 98.1° γ: 90°
R-values:
R R work R free
0.325 0.325 0.339
Expression system: Escherichia coli BL21(DE3)