5btf

X-ray diffraction
2.61Å resolution

Crystal structure of a topoisomerase II complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118443 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
DNA gyrase subunit A Chains: A, C
Molecule details ›
Chains: A, C
Length: 503 amino acids
Theoretical weight: 56.23 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WG47 (Residues: 2-500; Coverage: 60%)
Gene names: MTCY10H4.04, Rv0006, gyrA
Sequence domains: DNA gyrase/topoisomerase IV, subunit A
DNA gyrase subunit B Chains: B, D
Molecule details ›
Chains: B, D
Length: 253 amino acids
Theoretical weight: 28.27 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WG45 (Residues: 426-675; Coverage: 37%)
Gene names: MTCY10H4.03, Rv0005, gyrB
Sequence domains:
Structure domains: Rossmann fold
DNA substrate 24-mer GGTCATGAATGACTATGCACGTAA Chains: E, H
Molecule details ›
Chains: E, H
Length: 24 nucleotides
Theoretical weight: 7.42 KDa
DNA substrate 24-mer TTACGTGCATAGTCATTCATGACC Chains: F, G
Molecule details ›
Chains: F, G
Length: 24 nucleotides
Theoretical weight: 7.32 KDa

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 108.955Å b: 84.019Å c: 130.59Å
α: 90° β: 108.87° γ: 90°
R-values:
R R work R free
0.232 0.23 0.261
Expression systems:
  • Escherichia coli
  • Mycobacterium tuberculosis H37Rv