› 5b09

X-ray diffraction
1.7Å resolution

Polyketide cyclase OAC from Cannabis sativa bound with Olivetolic acid

Released:
DOI: 10.2210/pdb5b09/pdb
PDB entry 5b09 coloured by chain, front view.
PDB entry 5b09 coloured by chain, side view.
PDB entry 5b09 coloured by chain, top view.
Source organism: Cannabis sativa
Primary publication:
Structural basis for olivetolic acid formation by a polyketide cyclase from Cannabis sativa.
Yang X, Matsui T, Kodama T, Mori T, Zhou X, Taura F, Noguchi H, Abe I, Morita H
FEBS J 283 1088-106 (2016)
PMID: 26783002

Function and Biology Details

Reaction catalysed: 
3,5,7-trioxododecanoyl-CoA = CoA + 2,4-dihydroxy-6-pentylbenzoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125170 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Olivetolic acid cyclase Chain: A
Molecule details ›
Chain: A
Length: 104 amino acids
Theoretical weight: 12.23 KDa
Source organism: Cannabis sativa
Expression system: Escherichia coli
UniProt:
  • Canonical: I6WU39 (Residues: 1-101; Coverage: 100%)
Gene name: OAC
Sequence domains: Stress responsive A/B Barrel Domain
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
Ligand 4MX 1 x 4MX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: C2
Unit cell:
a: 86.48Å b: 30.44Å c: 36.89Å
α: 90° β: 108.47° γ: 90°
R-values:
R R work R free
0.198 0.197 0.223
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Phytocannabinoids: a unified critical inventory.
Hanuš et al. (2016)
8 more

3 mentions without citation

Bioengineering studies and pathway modeling of the heterologous biosynthesis of tetrahydrocannabinolic acid in yeast.
Thomas et al. (2020)
2 more