› 5ao2

X-ray diffraction
2.97Å resolution

Crystal structure of human SAMHD1 (amino acid residues 115-583) R164A variant bound to dGTP

Released:
DOI: 10.2210/pdb5ao2/pdb
PDB entry 5ao2 coloured by chain, front view.
PDB entry 5ao2 coloured by chain, side view.
PDB entry 5ao2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Phospho-dependent Regulation of SAMHD1 Oligomerisation Couples Catalysis and Restriction.
Arnold LH, Groom HC, Kunzelmann S, Schwefel D, Caswell SJ, Ordonez P, Mann MC, Rueschenbaum S, Goldstone DC, Pennell S, Howell SA, Stoye JP, Webb M, Taylor IA, Bishop KN
PLoS Pathog 11 e1005194 (2015)
PMID: 26431200

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195223 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 491 amino acids
Theoretical weight: 56.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y3Z3 (Residues: 115-583; Coverage: 75%)
Gene names: MOP5, SAMHD1
Sequence domains: HD domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand FE 4 x FE
Ligand DGT 4 x DGT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P21
Unit cell:
a: 69.706Å b: 199.32Å c: 81.804Å
α: 90° β: 100.79° γ: 90°
R-values:
R R work R free
0.16 0.156 0.234
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

From APOBEC to ZAP: Diverse mechanisms used by cellular restriction factors to inhibit virus infections.
Chemudupati et al. (2019)
12 more

1 mention without citation

Phospho-dependent Regulation of SAMHD1 Oligomerisation Couples Catalysis and Restriction.
Arnold et al. (2015)