› 5ajh

X-ray diffraction
1.9Å resolution

Crystal structure of Fusarium oxysporum cutinase

Released:
DOI: 10.2210/pdb5ajh/pdb
PDB entry 5ajh coloured by chain, front view.
PDB entry 5ajh coloured by chain, side view.
PDB entry 5ajh coloured by chain, top view.
Source organism: Fusarium oxysporum f. sp. raphani 54005
Primary publication:
Structural and functional studies of a Fusarium oxysporum cutinase with polyethylene terephthalate modification potential.
Dimarogona M, Nikolaivits E, Kanelli M, Christakopoulos P, Sandgren M, Topakas E
Biochim Biophys Acta 1850 2308-17 (2015)
PMID: 26291558

Function and Biology Details

Reaction catalysed: 
Cutin + H(2)O = cutin monomers
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197834 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cutinase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 226 amino acids
Theoretical weight: 23.12 KDa
Source organism: Fusarium oxysporum f. sp. raphani 54005
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: X0BTD8 (Residues: 17-230; Coverage: 100%)
Gene name: FOQG_13916
Sequence domains: Cutinase
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand MPD 6 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 35.906Å b: 60.141Å c: 241.965Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.181 0.224
Expression system: Escherichia coli BL21(DE3)

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Citations

11 review citations

Enzymatic Remediation of Polyethylene Terephthalate (PET)-Based Polymers for Effective Management of Plastic Wastes: An Overview.
Maurya et al. (2020)
10 more

5 mentions without citation

Perspectives on the Role of Enzymatic Biocatalysis for the Degradation of Plastic PET.
Magalhães et al. (2021)
4 more