› 5ae6

X-ray diffraction
2.1Å resolution

The structure of Hypocrea jecorina beta-xylosidase Xyl3A (Bxl1) in complex with 4-thioxylobiose

Released:
DOI: 10.2210/pdb5ae6/pdb
PDB entry 5ae6 coloured by chain, front view.
PDB entry 5ae6 coloured by chain, side view.
PDB entry 5ae6 coloured by chain, top view.
Source organism: Trichoderma reesei
Entry authors: Mikkelsen NE, Gudmundsson M, Karkehabadi S, Hansson H, Sandgren M, Larenas E, Mitchinson C, Keleman B, Kaper T

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187666 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (6 distinct):
xylan 1,4-beta-xylosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 767 amino acids
Theoretical weight: 84.26 KDa
Source organism: Trichoderma reesei
Expression system: Not provided
UniProt:
  • Canonical: Q92458 (Residues: 21-787; Coverage: 99%)
Gene name: bxl1
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: TXB, XYP
Ligand TXB 2 x TXB
Ligand XYP 2 x XYP
2 bound ligands:
Ligand NAG 10 x NAG
Ligand ZN 12 x ZN
1 modified residue:
Ligand PCA 2 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-2
Spacegroup: P21212
Unit cell:
a: 100.244Å b: 202.443Å c: 82.441Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.178 0.214
Expression system: Not provided

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