› 5a9i

X-ray diffraction
2.84Å resolution

Crystal structure of the extracellular domain of PepT2

Released:
DOI: 10.2210/pdb5a9i/pdb
PDB entry 5a9i coloured by chain, front view.
PDB entry 5a9i coloured by chain, side view.
PDB entry 5a9i coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Crystal Structures of the Extracellular Domain from PepT1 and PepT2 Provide Novel Insights into Mammalian Peptide Transport.
Beale JH, Parker JL, Samsudin F, Barrett AL, Senan A, Bird LE, Scott D, Owens RJ, Sansom MSP, Tucker SJ, Meredith D, Fowler PW, Newstead S
Structure 23 1889-1899 (2015)
PMID: 26320580

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179013 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Solute carrier family 15 member 2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 194 amino acids
Theoretical weight: 21.69 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q63424 (Residues: 410-601; Coverage: 26%)
Gene names: Pept2, Slc15a2

Ligands and Environments

2 bound ligands:
Ligand CIT 2 x CIT
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 9 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P3221
Unit cell:
a: 95.75Å b: 95.75Å c: 165.93Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.193 0.246
Expression system: Escherichia coli BL21(DE3)

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Citations

6 review citations

Recent advances in understanding proton coupled peptide transport via the POT family.
Newstead S. (2017)
5 more

1 mention without citation

The Structure and Mechanism of Drug Transporters.
Roberts AG. (2021)