› 5a2n

X-ray diffraction
3.7Å resolution

Crystal structure of the nitrate transporter NRT1.1 from Arabidopsis thaliana.

Released:
DOI: 10.2210/pdb5a2n/pdb
PDB entry 5a2n coloured by chain, front view.
PDB entry 5a2n coloured by chain, side view.
PDB entry 5a2n coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Molecular basis of nitrate uptake by the plant nitrate transporter NRT1.1.
Parker JL, Newstead S
Nature 507 68-72 (2014)
PMID: 24572366

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170125 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein NRT1/ PTR FAMILY 6.3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 590 amino acids
Theoretical weight: 64.98 KDa
Source organism: Arabidopsis thaliana
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q05085 (Residues: 1-590; Coverage: 100%)
Gene names: At1g12110, CHL1, F12F1.1, NPF6.3, NRT1, NRT1.1, T28K15_13
Sequence domains: POT family
Structure domains: MFS general substrate transporter like domains

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P212121
Unit cell:
a: 121.8Å b: 124.03Å c: 153.32Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.279 0.278 0.31
Expression system: Saccharomyces cerevisiae

Quick links

Citations

56 review citations

Structural Biology of the Major Facilitator Superfamily Transporters.
Yan N. (2015)
55 more

8 mentions without citation

MemProtMD: Automated Insertion of Membrane Protein Structures into Explicit Lipid Membranes.
Stansfeld et al. (2015)
7 more