› 4yyc

X-ray diffraction
1.56Å resolution

Crystal structure of trimethylamine methyltransferase from Sinorhizobium meliloti in complex with unknown ligand

Released:
DOI: 10.2210/pdb4yyc/pdb
PDB entry 4yyc coloured by chain, front view.
PDB entry 4yyc coloured by chain, side view.
PDB entry 4yyc coloured by chain, top view.
Source organism: Sinorhizobium meliloti 1021
Primary publication:
Protein purification and crystallization artifacts: The tale usually not told.
Niedzialkowska E, Gasiorowska O, Handing KB, Majorek KA, Porebski PJ, Shabalin IG, Zasadzinska E, Cymborowski M, Minor W
Protein Sci 25 720-33 (2016)
PMID: 26660914

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187901 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 525 amino acids
Theoretical weight: 57.87 KDa
Source organism: Sinorhizobium meliloti 1021
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q92P20 (Residues: 1-524; Coverage: 100%)
Gene names: SMc04330, mttB
Sequence domains: Trimethylamine methyltransferase (MTTB)
Structure domains: Trimethylamine methyltransferase-like

Ligands and Environments

2 bound ligands:
Ligand UNL 1 x UNL
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P21212
Unit cell:
a: 89.162Å b: 60.025Å c: 88.345Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.14 0.139 0.165
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

The impact of structural genomics: the first quindecennial.
Grabowski et al. (2016)
1 more

1 mention without citation

Protein purification and crystallization artifacts: The tale usually not told.
Niedzialkowska et al. (2016)