› 4m7w

X-ray diffraction
1.9Å resolution

Crystal structure of purine nucleoside phosphorylase from Leptotrichia buccalis C-1013-b, NYSGRC Target 029767.

Released:
DOI: 10.2210/pdb4m7w/pdb
PDB entry 4m7w coloured by chain, front view.
PDB entry 4m7w coloured by chain, side view.
PDB entry 4m7w coloured by chain, top view.
Source organism: Leptotrichia buccalis C-1013-b
Entry authors: Malashkevich VN, Bhosle R, Toro R, Hillerich B, Gizzi A, Garforth S, Kar A, Chan MK, Lafluer J, Patel H, Matikainen B, Chamala S, Lim S, Celikgil A, Villegas G, Evans B, Love J, Fiser A, Khafizov K, Seidel R, Bonanno JB, Almo SC, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Reaction catalysed: 
Uridine + phosphate = uracil + alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111730 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uridine phosphorylase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 258 amino acids
Theoretical weight: 28.45 KDa
Source organism: Leptotrichia buccalis C-1013-b
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C7N9Q9 (Residues: 1-236; Coverage: 100%)
Gene name: Lebu_0989
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

1 bound ligand:
Ligand PO4 3 x PO4
1 modified residue:
Ligand MSE 21 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P321
Unit cell:
a: 164.877Å b: 164.877Å c: 45.527Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.201 0.244
Expression system: Escherichia coli BL21(DE3)

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