› 4jj9

X-ray diffraction
1.76Å resolution

Crystal Structure of 5-carboxymethyl-2-hydroxymuconate delta-isomerase

Released:
DOI: 10.2210/pdb4jj9/pdb
PDB entry 4jj9 coloured by chain, front view.
PDB entry 4jj9 coloured by chain, side view.
PDB entry 4jj9 coloured by chain, top view.
Source organism: Bordetella bronchiseptica RB50
Entry authors: Eswaramoorthy S, Chamala S, Evans B, Foti F, Gizzi A, Hillerich B, Kar A, Lafleur J, Seidel R, Villigas G, Zencheck W, Al Obaidi N, Almo SC, Swaminathan S, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Reaction catalysed: 
5-carboxymethyl-2-hydroxymuconate = (3E,5R)-5-carboxy-2-oxohept-3-enedioate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102075 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5-carboxymethyl-2-hydroxymuconate delta-isomerase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 167 amino acids
Theoretical weight: 18.48 KDa
Source organism: Bordetella bronchiseptica RB50
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A0H3LHY8 (Residues: 1-145; Coverage: 100%)
Gene names: BB0738, hpaF
Sequence domains: 5-carboxymethyl-2-hydroxymuconate isomerase
Structure domains: Macrophage Migration Inhibitory Factor

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
1 modified residue:
Ligand MSE 15 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 60.345Å b: 75.481Å c: 107.645Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.212 0.234
Expression system: Escherichia coli BL21

Quick links