› 4ja3

X-ray diffraction
3.8Å resolution

Partially occluded inward open conformation of the xylose transporter XylE from E. coli

Released:
DOI: 10.2210/pdb4ja3/pdb
PDB entry 4ja3 coloured by chain, front view.
PDB entry 4ja3 coloured by chain, side view.
PDB entry 4ja3 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural basis for substrate transport in the GLUT-homology family of monosaccharide transporters.
Quistgaard EM, Löw C, Moberg P, Trésaugues L, Nordlund P
Nat Struct Mol Biol 20 766-8 (2013)
PMID: 23624861

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142832 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-xylose-proton symporter Chains: A, B
Molecule details ›
Chains: A, B
Length: 485 amino acids
Theoretical weight: 52.93 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AGF4 (Residues: 2-485; Coverage: 99%)
Gene names: JW3991, b4031, xylE
Sequence domains: Sugar (and other) transporter
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CD 1 x CD
Ligand LU 4 x LU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: C2
Unit cell:
a: 246.88Å b: 74.38Å c: 131.11Å
α: 90° β: 117.71° γ: 90°
R-values:
R R work R free
0.287 0.285 0.315
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Blood-Brain Barrier: From Physiology to Disease and Back.
Sweeney et al. (2019)
14 more

20 mentions without citation

GLUT, SGLT, and SWEET: Structural and mechanistic investigations of the glucose transporters.
Deng et al. (2016)
19 more