› 4zyg

X-ray diffraction
2.8Å resolution

Crystal structure of methylated Sulfolobus solfataricus O6-methylguanine methyltransferase

Released:
DOI: 10.2210/pdb4zyg/pdb
PDB entry 4zyg coloured by chain, front view.
PDB entry 4zyg coloured by chain, side view.
PDB entry 4zyg coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
Structure-function relationships governing activity and stability of a DNA alkylation damage repair thermostable protein.
Perugino G, Miggiano R, Serpe M, Vettone A, Valenti A, Lahiri S, Rossi F, Rossi M, Rizzi M, Ciaramella M
Nucleic Acids Res 43 8801-16 (2015)
PMID: 26227971

Function and Biology Details

Reaction catalysed: 
DNA (containing 4-O-methylthymine) + protein L-cysteine = DNA (without 4-O-methylthymine) + protein S-methyl-L-cysteine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188995 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methylated-DNA--protein-cysteine methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 151 amino acids
Theoretical weight: 17.07 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q97VW7 (Residues: 1-151; Coverage: 100%)
Gene names: SSO2487, ogt
Sequence domains: 6-O-methylguanine DNA methyltransferase, DNA binding domain
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SMC 2 x SMC

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P212121
Unit cell:
a: 48.486Å b: 50.223Å c: 142.026Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.191 0.29
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Targeting Genome Integrity in Mycobacterium Tuberculosis: From Nucleotide Synthesis to DNA Replication and Repair.
Miggiano et al. (2020)
2 more

4 mentions without citation

Every OGT Is Illuminated … by Fluorescent and Synchrotron Lights.
Miggiano et al. (2017)
3 more