› 4zv0

X-ray diffraction
1.4Å resolution

Function and Biology Details

Reaction catalysed: 
NAD(+) + H(2)O = ADP-D-ribose + nicotinamide
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191487 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
NAD(P)(+) glycohydrolase toxin Tse6 Chain: A
Molecule details ›
Chain: A
Length: 163 amino acids
Theoretical weight: 18.28 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9I739 (Residues: 282-430; Coverage: 35%)
Gene names: PA0093, tse6
Sequence domains: Bacterial toxin 46
Immune protein Tsi6 Chain: B
Molecule details ›
Chain: B
Length: 94 amino acids
Theoretical weight: 10.74 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9I740 (Residues: 1-94; Coverage: 100%)
Gene names: PA0092, tsi6
Sequence domains: Tsi6

Ligands and Environments

1 bound ligand:
Ligand IOD 10 x IOD
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P65
Unit cell:
a: 83.209Å b: 83.209Å c: 83.758Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.147 0.146 0.164
Expression system: Escherichia coli BL21

Quick links

Citations

26 review citations

An insider's perspective: Bacteroides as a window into the microbiome.
Wexler et al. (2017)
25 more

2 mentions without citation

An interbacterial NAD(P)(+) glycohydrolase toxin requires elongation factor Tu for delivery to target cells.
Whitney et al. (2015)
1 more