› 4zuv

X-ray diffraction
2.3Å resolution

Crystal structure of Equine MHC I(Eqca-N*00602) in complexed with equine infectious anaemia virus (EIAV) derived peptide Env-RW12

Released:
DOI: 10.2210/pdb4zuv/pdb
PDB entry 4zuv coloured by chain, front view.
PDB entry 4zuv coloured by chain, side view.
PDB entry 4zuv coloured by chain, top view.
Source organisms:
Primary publication:
Structural Illumination of Equine MHC Class I Molecules Highlights Unconventional Epitope Presentation Manner That Is Evolved in Equine Leukocyte Antigen Alleles.
Yao S, Liu J, Qi J, Chen R, Zhang N, Liu Y, Wang J, Wu Y, Gao GF, Xia C
J Immunol 196 1943-54 (2016)
PMID: 26764037

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-135108 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ig-like domain-containing protein Chains: A, D
Molecule details ›
Chains: A, D
Length: 274 amino acids
Theoretical weight: 31.6 KDa
Source organism: Equus caballus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q860N6 (Residues: 22-295; Coverage: 82%)
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, E
Molecule details ›
Chains: B, E
Length: 99 amino acids
Theoretical weight: 11.7 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Surface protein Chains: C, F
Molecule details ›
Chains: C, F
Length: 12 amino acids
Theoretical weight: 1.48 KDa
Source organism: Equine infectious anemia virus
Expression system: Not provided
UniProt:
  • Canonical: P16082 (Residues: 195-206; Coverage: 1%)
Gene name: env

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: P21
Unit cell:
a: 82.518Å b: 71.441Å c: 99.874Å
α: 90° β: 102.94° γ: 90°
R-values:
R R work R free
0.201 0.2 0.234
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

New vistas unfold: Chicken MHC molecules reveal unexpected ways to present peptides to the immune system.
Halabi et al. (2022)
12 other articles

1 mention without citation

Structural Definition of Duck Major Histocompatibility Complex Class I Molecules That Might Explain Efficient Cytotoxic T Lymphocyte Immunity to Influenza A Virus.
Wu et al. (2017)