› 4zp4

X-ray diffraction
2.36Å resolution

Crystal Structure of the Heterodimeric HIF-2a:ARNT Complex

Released:
DOI: 10.2210/pdb4zp4/pdb
PDB entry 4zp4 coloured by chain, front view.
PDB entry 4zp4 coloured by chain, side view.
PDB entry 4zp4 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural integration in hypoxia-inducible factors.
Wu D, Potluri N, Lu J, Kim Y, Rastinejad F
Nature 524 303-8 (2015)
PMID: 26245371

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156869 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Aryl hydrocarbon receptor nuclear translocator Chains: A, C
Molecule details ›
Chains: A, C
Length: 384 amino acids
Theoretical weight: 43.44 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P53762 (Residues: 81-464; Coverage: 49%)
Gene name: Arnt
Sequence domains:
Endothelial PAS domain-containing protein 1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 360 amino acids
Theoretical weight: 41.2 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P97481 (Residues: 3-361; Coverage: 41%)
Gene names: Epas1, Hif2a
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P1
Unit cell:
a: 49.006Å b: 76.463Å c: 98.817Å
α: 90.06° β: 90.19° γ: 73.13°
R-values:
R R work R free
0.203 0.2 0.232
Expression system: Escherichia coli BL21

Quick links

Citations

47 review citations

Transcriptional architecture of the mammalian circadian clock.
Takahashi JS. (2017)
46 more

21 mentions without citation

Structural characterization of mammalian bHLH-PAS transcription factors.
Wu et al. (2017)
20 more