› 4zp2

X-ray diffraction
2.2Å resolution

Crystal structure of E. coli multidrug transporter MdfA in complex with n-dodecyl-N,N-dimethylamine-N-oxide

Released:
DOI: 10.2210/pdb4zp2/pdb
PDB entry 4zp2 coloured by chain, front view.
PDB entry 4zp2 coloured by chain, side view.
PDB entry 4zp2 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Substrate-bound structure of the E. coli multidrug resistance transporter MdfA.
Heng J, Zhao Y, Liu M, Liu Y, Fan J, Wang X, Zhao Y, Zhang XC
Cell Res 25 1060-73 (2015)
PMID: 26238402

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142671 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multidrug transporter MdfA Chain: A
Molecule details ›
Chain: A
Length: 392 amino acids
Theoretical weight: 42.46 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P0AEY8 (Residues: 9-400; Coverage: 96%)
Gene names: JW0826, b0842, cmlA, cmr, mdfA
Sequence domains: Sugar (and other) transporter

Ligands and Environments

1 bound ligand:
Ligand LDA 2 x LDA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 94.086Å b: 64.085Å c: 101.253Å
α: 90° β: 104.19° γ: 90°
R-values:
R R work R free
0.208 0.206 0.232
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

22 review citations

Multidrug efflux pumps: structure, function and regulation.
Du et al. (2018)
21 more

9 mentions without citation

Structural Insights into Transporter-Mediated Drug Resistance in Infectious Diseases.
Kim et al. (2021)
8 more