› 4zl5

X-ray diffraction
1.85Å resolution

Crystal structure of the PmFTN variant E44H soaked in iron (45 min)

Released:
DOI: 10.2210/pdb4zl5/pdb
PDB entry 4zl5 coloured by chain, front view.
PDB entry 4zl5 coloured by chain, side view.
PDB entry 4zl5 coloured by chain, top view.
Source organism: Pseudo-nitzschia multiseries
Primary publication:
A Diatom Ferritin Optimized for Iron Oxidation but Not Iron Storage.
Pfaffen S, Bradley JM, Abdulqadir R, Firme MR, Moore GR, Le Brun NE, Murphy MEP
J Biol Chem 290 28416-28427 (2015)
PMID: 26396187

Function and Biology Details

Reaction catalysed: 
4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110318 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferritin Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 169 amino acids
Theoretical weight: 18.98 KDa
Source organism: Pseudo-nitzschia multiseries
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B6DMH6 (Residues: 63-230; Coverage: 80%)
Gene name: FTN
Sequence domains: Ferritin-like domain
Structure domains: Ferritin

Ligands and Environments

1 bound ligand:
Ligand FE 24 x FE
1 modified residue:
Ligand YCM 6 x YCM

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P4212
Unit cell:
a: 126.46Å b: 126.46Å c: 170.31Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.207
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Ferritin, cellular iron storage and regulation.
Arosio et al. (2017)
4 more

1 mention without citation

A Diatom Ferritin Optimized for Iron Oxidation but Not Iron Storage.
Pfaffen et al. (2015)