› 4ziv

X-ray diffraction
3.16Å resolution

Crystal structure of AcrB triple mutant in P21 space group

Released:
DOI: 10.2210/pdb4ziv/pdb
PDB entry 4ziv coloured by chain, front view.
PDB entry 4ziv coloured by chain, side view.
PDB entry 4ziv coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structures of Gate Loop Variants of the AcrB Drug Efflux Pump Bound by Erythromycin Substrate.
Ababou A, Koronakis V
PLoS One 11 e0159154 (2016)
PMID: 27403665

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151927 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multidrug efflux pump subunit AcrB Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 1049 amino acids
Theoretical weight: 113.43 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P31224 (Residues: 1-1049; Coverage: 100%)
Gene names: JW0451, acrB, acrE, b0462
Sequence domains: AcrB/AcrD/AcrF family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand LMT 6 x LMT
Ligand NI 3 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 152.278Å b: 157.491Å c: 219.155Å
α: 90° β: 92.74° γ: 90°
R-values:
R R work R free
0.273 0.27 0.335
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.
Alav et al. (2021)
8 more

2 mentions without citation

Structural Insights into Transporter-Mediated Drug Resistance in Infectious Diseases.
Kim et al. (2021)
1 more