› 4ziu

X-ray diffraction
2.7Å resolution

Crystal structure of native alpha-2-macroglobulin from Escherichia coli spanning the residues from domain MG7 to the C-terminus.

Released:
DOI: 10.2210/pdb4ziu/pdb
PDB entry 4ziu coloured by chain, front view.
PDB entry 4ziu coloured by chain, side view.
PDB entry 4ziu coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural and functional insights into Escherichia coli α2-macroglobulin endopeptidase snap-trap inhibition.
Garcia-Ferrer I, Arêde P, Gómez-Blanco J, Luque D, Duquerroy S, Castón JR, Goulas T, Gomis-Rüth FX
Proc Natl Acad Sci U S A 112 8290-5 (2015)
PMID: 26100869

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160110 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-2-macroglobulin Chain: A
Molecule details ›
Chain: A
Length: 639 amino acids
Theoretical weight: 69.62 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P76578 (Residues: 1018-1653; Coverage: 39%)
Gene names: JW2504, b2520, yfhM
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand NI 2 x NI
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC, ESRF BEAMLINE ID29
Spacegroup: I222
Unit cell:
a: 86.72Å b: 136.19Å c: 172.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.24
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Inhibitors and Antibody Fragments as Potential Anti-Inflammatory Therapeutics Targeting Neutrophil Proteinase 3 in Human Disease.
Korkmaz et al. (2016)
1 more

1 mention without citation

Structural and functional insights into Escherichia coli α2-macroglobulin endopeptidase snap-trap inhibition.
Garcia-Ferrer et al. (2015)