› 4z65

X-ray diffraction
1.25Å resolution

Carboxypeptidase B with Sulphamoil Arginine

Released:
DOI: 10.2210/pdb4z65/pdb
PDB entry 4z65 coloured by chain, front view.
PDB entry 4z65 coloured by chain, side view.
PDB entry 4z65 coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Structure of the complex of carboxypeptidase B and N-sulfamoyl-L-arginine.
Akparov V, Sokolenko N, Timofeev V, Kuranova I
Acta Crystallogr F Struct Biol Commun 71 1335-40 (2015)
PMID: 26457527

Function and Biology Details

Reaction catalysed: 
Preferential release of a C-terminal lysine or arginine amino acid.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140836 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carboxypeptidase B Chain: A
Molecule details ›
Chain: A
Length: 304 amino acids
Theoretical weight: 34.54 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: P09955 (Residues: 113-416; Coverage: 76%)
Gene names: CPB, CPB1
Sequence domains: Zinc carboxypeptidase
Structure domains: Zn peptidases

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand 0X9 1 x 0X9
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P41212
Unit cell:
a: 79.38Å b: 79.38Å c: 100.37Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.159 0.18
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Structure of the carboxypeptidase B complex with N-sulfamoyl-L-phenylalanine - a transition state analog of non-specific substrate.
Akparov et al. (2018)

1 mention without citation

Structure of the complex of carboxypeptidase B and N-sulfamoyl-L-arginine.
Akparov et al. (2015)