› 4z60

X-ray diffraction
2.5Å resolution

The 2.5-angstrom of crystal structure of Zn(2+)-bound PqqB from Pseudomonas Putida

Released:
DOI: 10.2210/pdb4z60/pdb
PDB entry 4z60 coloured by chain, front view.
PDB entry 4z60 coloured by chain, side view.
PDB entry 4z60 coloured by chain, top view.
Source organism: Pseudomonas putida KT2440
Entry authors: Tu X, Wilmot CM

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183513 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Coenzyme PQQ synthesis protein B Chain: A
Molecule details ›
Chain: A
Length: 308 amino acids
Theoretical weight: 33.99 KDa
Source organism: Pseudomonas putida KT2440
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q88QV5 (Residues: 1-303; Coverage: 100%)
Gene names: PP_0379, pqqB
Sequence domains: Beta-lactamase superfamily domain
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P43212
Unit cell:
a: 86.186Å b: 86.186Å c: 107.224Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.161 0.213
Expression system: Escherichia coli BL21(DE3)

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